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Lipids determined 24 h later by coincidence counting using a liquid were extracted from plasma membrane pellet preparations and scintillation counter model LS TD; Beckman Instruments, total free sterols and total phospholipids were measured as Fullerton, Calif. All sample counts were corrected for back- described previously Lester and Stein, Protein content ground counts and quenching.
Data are presented for each seg- was determined by the method of Bradford Vanadate ment as the percent of 45Ca measured in the total core sample i. Epidermal and hypodermal mesocarp tissue seg- Lipoxygenase activity in hy- fixed for at least 48 h in 1 formalin: After fixation, the segments were ously by Lester b.
One unit of lipoxygenase activity was dehydrated in a tertiary butyl alcohol series, embedded in Paraplast equal to 0. Oxford Labware, St. Louis, Mo. A mm-diameter cork stained with safranin and fast green, Johanson, Photomi- borer was used to cut core segments from harvested melons. Mineral Carl Zeiss Inc. Analysis of variance was used to evaluate treatment The hypodermal mesocarp section 5 mm thick was taken from differences for physicochemical and ion content data SAS Inst.
Data are expressed as the average of taken from the center of the mesocarp. All sections were weighed five, single-fruit replications per cultivar per treatment. Table 1. At 60z 2. Following 10 d cold storage, replications. Following 24 d storage, hypoder- and inner mesocarp tissues were similar on either a dry weight mal mesocarp tissue Ca concentrations were similar across most basis Table 1 or a fresh weight basis data not shown.
Mason et al.
Mill var. In our study, the surfactant declined during postharvest storage. Photomicrographs of tissue Ca concentrations were similar and as much as fold epidermal sections of netted muskmelon and honeydew fruit higher than those of middle or inner mesocarp tissues Table 2.
Table 2. Effect of postharvest application of Ca-chelate or Mg-chelate on Ca concentration of muskmelon mesocarp tissues.
Abscissed harvested fruit were dipped for 20 min in water, or a solution containing Ca-chelate 0. Tissue sectioning, processing, and Ca analyses were performed as described in the materials and methods. These results suggest that netting strongly impedes radial migration of ap- plied Ca-chelate in muskmelons. Higher Fig. Photomicrographs of longitudinal sections of epidermal and hypodermal concentrations of applied Ca-chelate may be needed for increased tissues from the equatorial regions of A d-old abscised netted muskmelons Ca penetration in netted melons.
However fruit Fig. Data are presented as a percent of the total 45Ca recovered in muskmelon epidermal and mesocarp regions within a core sample. Table 4. Effect of postharvest application of Ca-chelate or Mg-chelate on Mg concentration of muskmelon mesocarp tissues. Treatments are described in Table 2.
Table 5. Surface characteristics of muskmelons at harvest or following mineral chelate treatments and storage. See Table 2 for treatment details.
Table 6. Physical characteristics of muskmelons at abscission harvest or following mineral chelate treatments and storage. Calcium-chelate-treated fruit of 24 d storage. Peleg et al.
The the hypodermal mesocarp following 10 d storage. Tyler and exact regulation of muskmelon firmness loss is unknown, but is Lorenz compared Mg concentrations in four different thought to be associated with compositional changes in cell wall muskmelon types crenshaw, honeydew, netted muskmelon, and fractions McCollum et al.
Persian , and found that fruit Mg concentration changed very Our recent and current findings suggest that loss of musk- little with age. In nonevident. These fruit had higher Ca concentration in the the other treatments, also had the lowest TFS: Calcium is known to physical characteristics of fruit firmness, weight loss, and hypo- regulate the expression and synthesis of proteins and enzymes dermal mesocarp electrolyte leakage depending on duration of Poovaiah and Reddy, , and to reduce catabolism of total storage Table 6.
TPL Picchioni et al. TPL is one of the storage, had the lowest moisture loss and the least electrolyte most obvious changes which occurs during senescence, and is leakage following 24 d storage, compared to water or Mg-chelate due to catabolism of phospholipids Lester and Whitaker, Table 7.
Table 8. The decline in the Ca concentration in the hypoder- of Mg, thereby directly regulating melon PM permeability i. Kinoshita et al. In our study, Treating fully abscised, greenhouse-grown, hybrid honeydew muskmelons with elevated hypodermal mesocarp Ca concentra- muskmelons with 0. Inclusion of Ca-chelate dips with fected PM integrity. In netted high quality melons over greater distances, and thus to more and honeydew PM, lipid degradation via LOX activity is greatest markets.
Additionally, new markets could be developed for very in mature and postharvest fruit Lacan and Baccou, ; Lester, sweet, highly nutritious, vine ripened abscised honeydew fruit , b. Ferguson reported that Ca directly influ- which, at present, have a limited postharvest storage life.
This may have resulted from Ca indirectly cherry fruit. Acta Hort. Jean-Baptiste, G. Bertoni, and P. Changes tion, or by serving as a membrane protective barrier to disrupt in calcium contents during melon Cucumis melo L. Scientia Hort. In summary, this study demonstrated that Ca concentration in Bradford, M. A rapid and sensitive method for the quantitation muskmelon hypodermal mesocarp tissue is highest before abscis- of microgram quantities of protein utilizing the principle of protein dye sion.
Following 24 d postharvest storage, Ca concentration within binding. Postharvest treatment for control of stem-scar, rind,. Plant Dis. Clarkson, D. Calcium transport between tissues and its distribu- Lester, G. Gamma-ray-induced changes in tion in the plant. Plant Cell Environ. Calcium in plant senescence and fruit ripening.
Plant, Cell Environ. Mason, J. McDougald, and B. Calcium con- Garcia, J. Herrera, and A. Effects of postharvest centration in apple fruit resulting from calcium chloride dips modified dips in calcium chloride on strawberry. Food Chem. HortScience 9: Gerasopoulos, D. Chouliaras, and S. Effects of McCollum, T. Huber, and D. Modification of preharvest calcium chloride sprays on maturity and storability of polyuronides and hemicelluloses during muskmelon fruit softening.
Postharvest Biol. Grusak, M. Iron transport to developing ovules of Pisum Miklus, M. CaCl2 treated irrigation water sativum.
Seed import characteristics and phloem iron-loading capac- applied to mushroom crops Agaricus bisporus increases Ca concen- ity of source regions. Plant Physiol.
Mycologia Holman, R. Measurement of lipoxidase activity. Methods Bio- Peleg, K. Aharoni, A. Copel, and M. Non-destructive Johanson, A. Plant microtechnique. McGraw-Hill, New York. Nishimura, and K. Download gratis pdf Dongeng Favorit si Kancil. Februari 17, Dongeng Favorit si Kancil By: Baca gratis pdf Matahari kecil. Februari 05, Matahari kecil By: MizanNovel Anak Islami adalah fiksi untuk anak-anak yang dikemas dengan bahasa yang ringan, tetai sarat dengan nilai-nilai Islam.
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