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D On the last day 14th day , the above ground plant part was collected and fresh weight was measured Precisa All AG inoculated plants were checked for disease symptoms. For the control P treatment Supplementary Table 1 , plants without previous aphid infestation were inoculated with AG This experiment was repeated as three independent experimental replicates at different times over 6 months. Compost from two biological replicate plants were combined into one sample 2 g for each treatment of each variety. DNA was extracted using the method described by Ray et al. The amount of DNA was then determined by linear regression. MYC2 transcription factor regulates cross talk in the JA signaling pathway and defenses against herbivory Kazan and Manners, ERF1 transcription factor is activated by coordination of ET and JA signaling pathways and regulates the expression of genes against necrotrophic pathogens Kazan and Manners, Collection of Samples Based on results of experiments on the effect of M. Plants were grown, infested with M. Samples were collected from five different treatments: Aphid A , pathogen P , and aphid with pathogen AP and two control treatments control 1 and control 2. We chose two time points to examine an early and a later stage of infection earlier observations showed that at 72 h AG hyphae reach the plant. Time points were taken at 24 h intervals to exclude the effect of circadian cycle in the expression of genes.

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